![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K1834847:Design
Designed by: Gerry Bourassa Group: iGEM15_consort_alberta (2015-09-08)
Xylene Sensor! With AmilCP!
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2196 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 147
Illegal BglII site found at 1231
Illegal BglII site found at 1609 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 998
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 851
Design Notes
We optimized everything up until the AmilCP (this can be found looking at the sequence on the Main Page. Optimized DNA is CAPATALIZED, AmilCP is lowercase). Illegal PST1 restriction site present in theXylR gene was removed. (See part K1834844)
Source
E. Coli
References
http://2015.igem.org/Team:Consort_Alberta/project