Composite

Part:BBa_K1834847:Design

Designed by: Gerry Bourassa   Group: iGEM15_consort_alberta   (2015-09-08)

Xylene Sensor! With AmilCP!


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2196
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
    Illegal BglII site found at 1231
    Illegal BglII site found at 1609
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 998
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 851


Design Notes

We optimized everything up until the AmilCP (this can be found looking at the sequence on the Main Page. Optimized DNA is CAPATALIZED, AmilCP is lowercase). Illegal PST1 restriction site present in theXylR gene was removed. (See part K1834844)


Source

E. Coli

References

http://2015.igem.org/Team:Consort_Alberta/project